Lambda terminase
TīmeklisThe maturation and packaging of bacteriophage lambda DNA are catalyzed by the phage terminase enzyme. Terminase is composed of two protein subunits, gpNu1 … TīmeklisThe terminase large subunit acts as an ATP driven molecular motor necessary for viral DNA translocation into empty capsids and as an endonuclease that cuts the viral …
Lambda terminase
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TīmeklisThe role of cosB, the binding site for terminase, the DNA packaging enzyme of bacteriophage lambda, in the nicking reaction. J Mol Biol … TīmeklisA key player in the lambda DNA packaging process is the phage-encoded enzyme terminase, which is involved in (i) recognition of the concatemeric lambda DNA; (ii) initiation of packaging, which includes the introduction of staggered nicks at cosN to generate the cohesive ends of virion DNA and the binding of the prohead; (iii) DNA …
Tīmeklis2001. gada 10. aug. · An initiation-defective mutant form of cosBsponsored efficient processivity, indicating that the terminase-cosB interactions required for termination are less stringent than those required at initiation. ... the large subunit of bacteriophage lambda terminase, inactivate the endonuclease activity of terminase. J. Mol. Biol. … Tīmeklis2000. gada 20. janv. · This review focuses on the biochemical, biophysical, and catalytic properties of terminase, an enzyme involved in bacteriophage lambda genome …
Tīmeklis2000. gada 20. janv. · It is demonstrated that lambda terminase can be purified as a homogeneous "protomer" species, and a kinetic analysis of the genome maturation and packaging activities of the protomeric enzyme is presented, providing mechanistic insight into the coordinated catalytic activities of terminase enzymes in virus … Tīmeklis1999. gada 20. dec. · Terminase, the DNA packaging enzyme of bacteriophage lambda, is a heteromultimer composed of gpNu1 (181 aa) and gpA (641 aa) …
TīmeklisThe extents of the sites for nicking (cosN) and binding (cosB) of bacteriophage lambda DNA by terminase have been determined by studying cos cleavage and terminase binding in vitro. The cosN site is located in the segment from -22 to +24 bp (numbered from the center of the cohesive end sequence in t …
Tīmeklis2013. gada 25. marts · Terminase enzymes catalyze viral genome packaging. All characterized terminases are composed of large (TerL) and small (TerS) subunits in hetero-oligomeric complexes, although the subunit stoichiometry in the functional motors remains ill defined in most cases (3, 6).Most of the enzymes also possess a DNA … different types of materials and their usesTīmeklis2014. gada 1. janv. · The cos site allows the recombinant plasmid DNA to be cleaved by lambda terminase and packaged into phage particles which can be introduced very efficiently into host cells by the phage infection process (Collins and Hohn 1978). Cosmids can accommodate DNA inserts of 35–45 kb, larger than are easily handled … different types of materials for green wallTīmeklis[03-28] 国家水禽产业技术体系疾病防控研究室2024年 [03-20] bx795抗鸭瘟病毒感染宿主细胞机制 [03-14] 一种快速鉴别鸭瘟强、弱毒的pcr 方法的建立 [03-02] 四川农业大学预防兽医研究所首次发现革兰氏 [02-28] gn蛋白影响鸭瘟病毒感染宿主细胞吗？ [02-06] 国家农业产业技术体系四川省兽药创新团队20 form mo-ms 2020Tīmeklis2012. gada 25. okt. · Here, we demonstrate that lambda terminase can be purified as a homogeneous “protomer” species, and we present a kinetic analysis of the genome maturation and packaging activities of the protomeric enzyme. The protomer assembles into a distinct maturation complex at the cos sequence of a concatemer. form modes powerappsTīmeklis1997. gada 13. maijs · The terminase enzyme from bacteriophage λ is responsible for excision of a single genome from a concatameric DNA precursor and its insertion into an empty viral procapsid. The enzyme possesses a site-specific endonuclease activity which is responsible for excision of the viral genome and the formation of the 12 … different types of materials for hatsTīmeklisTerminase is composed of two protein subunits, gpNu1 and gpA. The holoenzyme is multifunctional in vitro; it binds to and cleaves lambda DNA at the cos site (where cos represents cohesive-end site), packages DNA into lambda proheads, and is also a DNA-dependent ATPase. different types of material strengthTīmeklis1988. gada 1. jūl. · The large subunit of phage lambda terminase, gpA, the gene product of the phage A gene, interacts with the small subunit, gpNul, to form functional terminase. Terminase binds to lambda DNA at cosB to form a binary complex. The terminase:DNA complex binds a prohead to form a ternary complex. Ternary … different types of materials for clothes